Adsorbable Organic Fluorine (AOF) testing, performed in accordance with EPA Method 1621, is a screening technique used to measure the total concentration of organically bound fluorine present in aqueous samples. This method is commonly used as an indicator of potential PFAS contamination without targeting individual PFAS compounds.
Unlike compound-specific PFAS analyses, AOF provides a single total fluorine result, making it an effective tool for early-stage screening, compliance evaluations, and product development decision-making.
PFAS compounds contain strong carbon–fluorine bonds that resist degradation and can persist in products and the environment. AOF testing helps manufacturers and regulators:
Screen samples for total organic fluorine
Identify samples that may require follow-up PFAS analysis
Reduce costs compared to broad PFAS compound panels
Support regulatory risk assessments and compliance strategies
AOF is particularly useful when the presence or absence of fluorinated organic compounds is more important than identifying individual PFAS species.
EPA Method 1621 measures organic fluorine using the following general process:
Adsorption of organic fluorine compounds onto activated carbon
Removal of inorganic fluoride through washing steps
Combustion of the activated carbon to convert organic fluorine to hydrogen fluoride
Detection and quantification of fluoride using ion chromatography
The final result is reported as total adsorbable organic fluorine, typically expressed in µg/L as F.
Adsorbable Organic Fluorine testing is commonly used for:
PFAS screening assessments
Water and wastewater monitoring
Consumer product development support
Regulatory and compliance investigations
Prioritization of samples for targeted PFAS testing
Comparative studies across formulations or lots
| AOF Testing | Targeted PFAS Testing |
|---|---|
| Measures total organic fluorine | Measures individual PFAS compounds |
| Faster and more cost-effective | Higher analytical specificity |
| Ideal for screening | Ideal for confirmatory analysis |
| Non-compound specific | Compound-specific results |
Many clients use AOF testing as a first-step evaluation, followed by targeted PFAS analysis if elevated fluorine levels are detected.
Aqueous product extracts
Water-based formulations
Rinse waters and leachates
Environmental water samples
If your sample is not clearly aqueous, Pentyl Labs can help determine suitability or recommend alternative testing approaches.
Pentyl Labs provides Adsorbable Organic Fluorine testing with a focus on:
Practical regulatory guidance
Clear, decision-ready reporting
Fast turnaround options
Integration with PFAS and Total Organic Fluorine testing strategies
Our team helps clients understand what the data means, not just what the number is.
PFAS Testing
Total Organic Fluorine (TOF)
Regulatory Compliance Testing
Consumer Product Safety Screening